Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: Redox Biology

Article Title: Exercise and dietary intervention ameliorate high-fat diet-induced NAFLD and liver aging by inducing lipophagy

doi: 10.1016/j.redox.2020.101635

Figure Lengend Snippet: Exercise increased the FGF21 levels in serum and muscle. (A) FGF21 level in serum by ELISA. (B) FGF21 protein levels in liver and muscle by Western Blot. (C) FGF21 content in liver and muscle by ELISA. (D) FGF21 mRNA levels in liver and muscle by qPCR. Data are shown as the means ± SEM, n = 8 per group. Significance was designated by asterisks with *p < 0.05.

Article Snippet: Rat FGF21 enzyme-linked immunosorbent assay (ELISA) kit was obtained from Cusabio Biotech (Cat. No. CSB-EL008627RA, Cusabio Biotech, Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Redox Biology

Article Title: Exercise and dietary intervention ameliorate high-fat diet-induced NAFLD and liver aging by inducing lipophagy

doi: 10.1016/j.redox.2020.101635

Figure Lengend Snippet: FGF21 attenuated LDs accumulation in FFA-treated HepG2 cells through an autophagy dependent way. HepG2 cells were treated with FFA (400 μM). (A) Representative confocal images of LDs by BODIPY 493/503 staining. Scale: 50 μm. (B) TG level in HepG2 cells. (C) Representative immunoblotting of PLIN2, LC3II/LC3I and p62 in HepG2 cells. HepG2 cells were pre-transfected with Atg5 shRNA or empty vector by lenti-virus, then treated with FFA (400 μM) with or without FGF21 (1 μg/ml). (D) Representative confocal images of LDs by BODIPY 493/503 staining. Scale: 50 μm. (E) TG level in HepG2 cells. Experiments were repeated three times. Data are shown as the means ± SEM. Significance was designated by asterisks with *p < 0.05.

Article Snippet: Rat FGF21 enzyme-linked immunosorbent assay (ELISA) kit was obtained from Cusabio Biotech (Cat. No. CSB-EL008627RA, Cusabio Biotech, Wuhan, China).

Techniques: Staining, Western Blot, Transfection, shRNA, Plasmid Preparation, Virus

Journal: Redox Biology

Article Title: Exercise and dietary intervention ameliorate high-fat diet-induced NAFLD and liver aging by inducing lipophagy

doi: 10.1016/j.redox.2020.101635

Figure Lengend Snippet: FGF21 increased lipophagy through AMPK-dependent pathway in FFA-treated HepG2 cells. HepG2 cells were treated with FFA (400 μM), FGF21 (1 μg/ml) as well as AMPK inhibitor-Dorsomorphin (1 μmol/L). (A) Representative of p -AMPK (Thr172)/AMPK total, p -ULK1 (Ser555)/ULK1 total and LC3-II/LC3-I immunoblotting in HepG2 cells treated with FFA, FGF21 and Dorsomorphin, and the quantified data. (B) Representative confocal images of HepG2 cells expressing GFP-RFP-LC3 and quantitation of early autophagosome puncta and autolysosome puncta following FFA, FGF21 and Dorsomorphin treatment. Yellow showed co-localization of GFP and RFP, indicating early autophagosomes. Red only showed autolysosomes, Scale: 20 μm. Experiments were repeated three times. Data are shown as the means ± SEM. Significance was designated by asterisks with *p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Rat FGF21 enzyme-linked immunosorbent assay (ELISA) kit was obtained from Cusabio Biotech (Cat. No. CSB-EL008627RA, Cusabio Biotech, Wuhan, China).

Techniques: Western Blot, Expressing, Quantitation Assay

Journal: Redox Biology

Article Title: Exercise and dietary intervention ameliorate high-fat diet-induced NAFLD and liver aging by inducing lipophagy

doi: 10.1016/j.redox.2020.101635

Figure Lengend Snippet: FGF21 increased the lipophagy and reduced the lipid accumulation in FFA-treated HepG2 cells. HepG2 cells were treated with FFA (400 μM), FGF21 (1 μg/ml) as well as AMPK inhibitor-Dorsomorphin (1 μmol/L). (A) Representative confocal images of colocalization of LDs and autophagosomes by BODIPY 493/503 staining in GFP-LC3 protein expressed HepG2 cells. Scale: 50 μm. (B) TG level in HepG2 cells treated with FFA, FGF21 and Dorsomorphin. Experiments were repeated three times. Data are shown as the means ± SEM. Significance was designated by asterisks with *p < 0.05.

Article Snippet: Rat FGF21 enzyme-linked immunosorbent assay (ELISA) kit was obtained from Cusabio Biotech (Cat. No. CSB-EL008627RA, Cusabio Biotech, Wuhan, China).

Techniques: Staining

Journal: Redox Biology

Article Title: Exercise and dietary intervention ameliorate high-fat diet-induced NAFLD and liver aging by inducing lipophagy

doi: 10.1016/j.redox.2020.101635

Figure Lengend Snippet: FGF21 ameliorated the cell senescence through AMPK dependent autophagic pathway in FFA-treated WI-38 cells. WI-38 cells were treated with FFA (400 μM), FGF21 (1 μg/ml) as well as AMPK inhibitor-Dorsomorphin (1 μmol/L). (A) Representative of p -AMPK (Thr172)/AMPK total, p -ULK1 (Ser555)/ULK1 total and LC3-II/LC3-I immunoblotting in WI-38 cells and quantified data. (B) TG level in WI-38 cells. (C) Representative SA-β-gal staining (Scale: 50 μm), (D) SA-β-gal activity, (E) lipofuscin and (F) MDA contents in WI-38 cells. (G) p16 and (H) p27 mRNA levels in WI-38 cells. Experiments were repeated three times. Data are shown as the means ± SEM. Significance was designated by asterisks with *p < 0.05.

Article Snippet: Rat FGF21 enzyme-linked immunosorbent assay (ELISA) kit was obtained from Cusabio Biotech (Cat. No. CSB-EL008627RA, Cusabio Biotech, Wuhan, China).

Techniques: Western Blot, Staining, Activity Assay

Journal: BMJ Open

Article Title: Association between serum vitamin B 6 concentration and risk of osteoporosis in the middle-aged and older people in China: a cross-sectional study

doi: 10.1136/bmjopen-2018-028129

Figure Lengend Snippet: Comparison of serum Vit B 6 and serological metabolism parameters among control, osteopenia and osteoporosis groups

Article Snippet: The concentration of fibroblast growth factor 23 (FGF23) was measured through an ELISA performed using a human FGF23 ELISA kit (CSB-E10113h, Cusabio Biotech, Wuhan, China) and a micro plate reader (MK3, Thermo, Waltham, Massachusetts, USA).

Techniques: Comparison, Control

Journal: BMJ Open

Article Title: Association between serum vitamin B 6 concentration and risk of osteoporosis in the middle-aged and older people in China: a cross-sectional study

doi: 10.1136/bmjopen-2018-028129

Figure Lengend Snippet: Relationship between serum Vit B 6 concentrations and bone metabolism parameter concentrations in control, osteopenia and osteoporosis groups

Article Snippet: The concentration of fibroblast growth factor 23 (FGF23) was measured through an ELISA performed using a human FGF23 ELISA kit (CSB-E10113h, Cusabio Biotech, Wuhan, China) and a micro plate reader (MK3, Thermo, Waltham, Massachusetts, USA).

Techniques: Control