Journal: eLife

Article Title: The spatial separation of processing and transport functions to the interior and periphery of the Golgi stack

doi: 10.7554/eLife.41301

Figure Lengend Snippet:

Article Snippet: Cell line ( Rattus norvegicus ) , Normal rat kidney (NRK) fibroblast cell , ATCC , ATCC: CRL-1570; RRID: CVCL_2144 , .

Techniques: Recombinant, Plasmid Preparation, Antibody Labeling, Software

Journal: Advanced Science

Article Title: Copper‐loaded Milk‐Protein Derived Microgel Preserves Cardiac Metabolic Homeostasis After Myocardial Infarction

doi: 10.1002/advs.202401527

Figure Lengend Snippet: The effects of CuCMG on cardiomyocytes, endothelial cells, and fibroblasts. A) Schematic diagram of OGD model building, dosing, and detection times. B) JC‐1 staining of H9C2 cells from Blank, Control, CMG, Cu, and CuCMG groups. n = 4. Scale bars = 100 µm. C) Statistical analysis of the JC‐1 staining. D) The ATP levels of H9C2 cells in different groups after OGD treatment. E) qRT‐PCR analysis of the expression levels of HIF1A , VEGFA , and FGF2 in HUVECs treated with CMG and CuCMG separately. F) qRT‐PCR analysis of the expression levels of Postn , Col1a1 , and Ccn2 in pre‐induced MCFs treated with CMG and CuCMG separately. one‐way ANOVA was used for statistical analyses. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. ns, no statistical significance.

Article Snippet: Isolation of myocardial fibroblasts (MCFs) from neonatal mice was performed following literature using a Neonatal Cardiac Fibroblast Isolation Kit (Miltenyi, Germany).

Techniques: Staining, Control, Quantitative RT-PCR, Expressing

Journal:

Article Title: Transcriptional and Posttranscriptional Inhibition of Lysyl Oxidase Expression by Cigarette Smoke Condensate in Cultured Rat Fetal Lung Fibroblasts

doi: 10.1093/toxsci/kfi212

Figure Lengend Snippet: RFL6 cells were co-transfected with LO promoter-reporter plasmids and pSV-β-galactosidase (β-gal) vectors. After 6 h posttransfection, cells were washed, incubated in 10% FBS/DMEM for an additional 18 h period, washed again, and exposed to CSC at indicated doses for 24 h. Luciferase and β-gal activities in control and treated cells were measured according to manufacturer’s instructions. LO-promoter activities in transfected cells were normalized to β-gal activities and expressed as cpm/OD β-gal (100% luciferase activity in the control = 3,528 ± 312 cpm/OD β-gal). ** p < 0.01 vs. control for cells treated with 80 and 120 μg/ml of CSC .

Article Snippet: Rat fetal lung fibroblasts (RFL6) obtained from ATCC were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) as previously described ( Chen et al ., 2005 ).

Techniques: Transfection, Incubation, Luciferase, Control, Activity Assay

Journal: Cell Transplantation

Article Title: c-Casitas b-Lineage Lymphoma Downregulation Improves the Ability of Long-term Cultured Mesenchymal Stem Cells for Promoting Angiogenesis and Diabetic Wound Healing

doi: 10.1177/0963689721989605

Figure Lengend Snippet: The migration and angiogenic factors paracrine activity were decreased in long-term cultured MSCs and increased by treating with c-Cbl LNA Gapmers. (A) HUVECs were suspended in EC complete medium (PC) or vehicle (NC)-, P3 MSCs-, P10 MSCs-P10 + scramble LNA Gapmers (P10 + SCR) MSCs-, P10 + c-Cbl LNA Gapmers (P10 + c-Cbl KD) MSCs-conditioned EC basal medium supplemented with 0.75% FBS, and incubated for 8 h (original magnification, 10×) ( n = 3). The tube length, nodes, and meshes in different groups counted in three fields per well were statistically analyzed by histogram analysis. (B) The VEGFA, HGF, and bFGF level in the conditioned media were assessed by ELISA assay ( n = 3). (C) The representative picture and histogram analysis of the stained MSCs migrated to the bottom surface of the upper chamber with 10× magnification in each group ( n = 3). Error bars represent the mean ± SE. # P < 0.05 compared with the NC group and * P < 0.05 versus between the indicated groups. bFGF: basic fibroblast growth factor; c-Cbl: c-Casitas b-lineage lymphoma; EC: endothelial cell; HGF: hepatocyte growth factor; HUVEC: human umbilical vein endothelial cell; LNA Gapmers: locked nucleic acid–modified antisense oligonucleotide gapmers; MSC: mesenchymal stem cell; NC: negative control; PC: positive control; SE: standard error; VEGFA: vascular endothelial growth factor A.

Article Snippet: The supernatant was absorbed and the amount of angiogenic factors was analyzed by vascular endothelial growth factor A (VEGFA) ELISA kit (BOSTER Biological Technology, Wuhan, China), basic fibroblast growth factor (bFGF) ELISA kit (Cusabio Biotech, Wuhan, China), and hepatocyte growth factor (HGF) ELISA kit (BOSTER Biological Technology, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Migration, Activity Assay, Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Staining, Modification, Negative Control, Positive Control

Journal: eLife

Article Title: High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss

doi: 10.7554/elife.52709

Figure Lengend Snippet: Figure 6. Phytate-mediated Ca2+ deficiency promotes vitamin D insufficiency and renal phosphate wasting independent of FGF23 expression. (A–C) Time-course analysis of serum levels of intact PTH (A), 25(OH)D (B), and 1,25(OH)2D (C) in rats fed control, HP-LCa2+, and HP-HCa2+ diets. For the early measurement of 25(OH)D (B) and 1,25(OH)2D, we pooled the sera from 2 to 3 rats. (D–F) Time-course analysis of renal CYP27B1 (D), CYP24A1 (E), and VDR (F) in rats fed control, HP-LCa2+, and HP-HCa2+ diets. (G) Immunoblot analysis of renal aKlotho, NHERF1, NaPi-2a in rats fed control, HP-LCa2+, Figure 6 continued on next page

Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Rattus norvegicus) Sprague Dawley rat Orient Bio, Seoul, Korea Developed by Sprague Dawley, Inc. Chemical compound, drug AIN-93G Dyets Inc. DYET# 10700 Control diet Chemical compound, drug Phytic acid Sigma-Aldrich Cat. #: P-8810 Phytate diet Chemical compound, drug HNO3 Sigma-Aldrich Cat. #: 438073 Fecal mineral analysis Chemical compound, drug HF Sigma-Aldrich Cat. #: 695068 Fecal mineral analysis Chemical compound, drug HCl Sigma-Aldrich Cat. #: H1758 Fecal mineral analysis Chemical compound, drug EDTA Sigma-Aldrich Cat. #: 93283 Fecal phytate analysis Chemical compound, drug NaOH Sigma-Aldrich Cat. #: 415413 Fecal phytate analysis Chemical compound, drug Calcium Beckman Coulter OSR6113 Serum/urine biochemistry Chemical compound, drug Phosphate Beckman Coulter OSR6122 Serum/urine biochemistry Chemical compound, drug BUN (Urea) Beckman Coulter OSR6134 Serum/urine biochemistry Chemical compound, drug Creatinine Beckman Coulter OSR6178 Serum/urine biochemistry Chemical compound, drug Magnesium Beckman Coulter OSR6189 Serum/urine biochemistry Chemical compound, drug Urine protein Beckman Coulter OSR6170 Urine biochemistry Commercial assay or kit Rat intact PTH Immutopics Cat. #: 60–2500 ELISA kit Commercial assay or kit Human PTH Abcam Cat. #: ab230931 ELISA kit Commercial assay or kit Rat soluble RANKL Immundiagnostik Cat. #: K1019 ELISA kit Commercial assay or kit Rat osteoprotegerin Alpco Immunoassay Cat. #: 30–1020 ELISA kit Commercial assay or kit Rat intact FGF23 Elabscience Cat. #: E-EL-R3031 ELISA kit Commercial assay or kit Rat C-terminal FGF23 Elabscience Cat. #: E-EL-RB0377 ELISA kit Commercial assay or kit Human FGF23 Elabscience Cat. #: E-EL-H1116 ELISA kit Commercial assay or kit 25(OH)-Vitamin D Abbott/USA ARCHITECT 25-OH Vitamin D Chemiluminescence microparticle immunoassay Commercial assay or kit 1,25-(OH)two vitamin D DIAsoure 1,25(OH)2-VIT, D-RIA-CT/Belgium Radioimmunoassay Continued on next page Kim et al. eLife 2020;9:e52709.

Techniques: Expressing, Control, Western Blot